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AIM:To study the activity of telomerase and theexpression of human telomerase reverse transcriptase(hTERT)in colorectal carcinoma and its adjacent tissues,normal mucosa and adenomatoid polyp,and to evaluatetheir relation with carcinogenesis and progression ofcolorectal carcinoma.METHODS:Telomerase activity and hTERT expressionwere determined in 30 samples of colorectal carcinomaand its adjacent tissues,normal mucosa and 20samples of adenomatoid polyp by modified telomericrepeat amplification protocol(TRAP),enzyme-linkedimmunosorbent assay(ELISA)and immunohistochemicalmethod.RESULTS:Telomerase activity and hTERT expressionwere 83.33%(25/30)and 76.67%(23/30)respectivelyin colorectal carcinoma,which were obviously higherthan those in paracancerous tissues(13.33%,16.67%),normal mucosa(3.33%,3.33%)and adenomatoid polyp(10%,10%).There was a significant difference betweencolorectal carcinoma and other tissues(P=0.027).Thetelomerase activity and hTERT expression were higher incolorectal carcinoma with lymphatic metastasis than inthat without lymphatic metastasis(P=0.034).When thehistological classification and clinical stage were greater,the telomerase activity and hTERT expression increased,but there was no significant difference between them.In colorectal carcinoma,the telomerase activity wascorrelated with hTERT expression(positive vs negative expression of telomerase activity and hTERT,P=0.021).
AIM: To study the activity of telomerase and the expression of human telomerase reverse transcriptase (hTERT) in colorectal carcinoma and its adjacent tissues, normal mucosa and adenomatoid polyp, and to evaluate the relationship with carcinogenesis and progression of colorectal carcinoma. METHODS: Telomerase activity and hTERT expressionwere determined in 30 samples of colorectal carcinoma and its adjacent tissues, normal mucosa and 20samples of adenomatoid polyp by modified telomeric repeat amplification protocol (TRAP), enzyme-linked immunosorbent assay (ELISA) and immunohistochemical method. RESULTS: Telomerase activity and hTERT expressionwere 83.33% (25/30 ) and 76.67% (23/30) respectivelyin colorectal carcinoma, which were obviously higherthan those in paracancerous tissues. (13.33%, 16.67%), normal mucosa (3.33%, 3.33%) and adenomatoid polyp was a significant difference betweencolorectal carcinoma and other tissues (P = 0.027) .Thetelomerase activity and hTERT expression were higher incolorectal carcin oma with lymphatic metastasis than inthat without lymphatic metastasis (P = 0.034) .When thehistological classification and clinical stage were greater, the telomerase activity and hTERT expression increased, but there was no significant difference between them.In colorectal carcinoma, the telomerase activity wascorrelated with hTERT expression (positive vs negative expression of telomerase activity and hTERT, P = 0.021).