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应用小剂量孕马血清促性腺激素ＰＭＳＣ处理未成年雌性大鼠建立的卵泡闭锁模型，应用荧光分光光度计和流式细胞仪测定了大鼠颗粒细胞膜流动性、丙二醛（ＭＤＡ）含量和线粒体膜电位：在此基础上，进一步观察酪氨酸（Ｔｙｒ）、米非司酮对它们的影响。结果表明：与发育中的卵泡相比，卵泡闭锁时，颗粒细胞膜流动性明显降低（Ｐ＜０．００２）、膜ＭＤＡ水平明显升高（Ｐ＜０．００１），线粒体膜电位明显下降（Ｐ＜０．００１）。与实验组相比，酪氨酸使膜流动性升高，ＭＤＡ水平下降，线粒体腹电位升高，但都无统计学意义（Ｐ＞０．０５）；而米非司酮使膜流动性明显下降（Ｐ＜０．０１），ＭＤＡ水平明显升高（Ｐ＜０．０５），线粒体膜电位进一步下降（Ｐ＜０．０１）。研究结果表明，颗粒细胞膜ＭＤＡ水平是影响膜流动性的一个重要因素，膜脂质过氧化及线粒体膜电位下降在卵泡闭锁中也起着重要的作用。
The small-dose pregnant mare serum gonadotropin-PMSC was used to treat the atresia model of ovarian follicles, and the flow cytometry, the content of malondialdehyde (MDA) and mitochondria Membrane potentials: On this basis, to further observe the tyrosine (Tyr), mifepristone on them. The results showed that compared with the developing follicles, the fluidity of granular cell membrane was significantly decreased (P <0.002), the membrane MDA level was significantly increased (P <0.001) and mitochondrial membrane potential was significantly decreased (P <0.001). Compared with the experimental group, tyrosine increased membrane fluidity, decreased MDA level and increased mitochondrial abdominal potential (P> 0.05), while mifepristone significantly increased membrane fluidity (P <0.01), MDA level increased significantly (P <0.05) and mitochondrial membrane potential decreased further (P <0.01). The results showed that the level of MDA in granular cell membrane was an important factor affecting membrane fluidity. The decrease of membrane lipid peroxidation and mitochondrial membrane potential also played an important role in follicular atresia.