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目的:对黄芩中4种主要黄酮成分进行抗抑郁活性筛选。方法:雄性ICR小鼠随机分为10组,分别为:空白对照组、阳性对照氟西汀组(20 mg.kg-1)、黄芩苷、黄芩素、汉黄芩苷和汉黄芩素治疗组,其中4种黄酮各自分为10 mg.kg-1和20 mg.kg-12个剂量组。连续ig给药7 d。末次给药1 h后,分别采用强迫游泳、悬尾和开场实验对小鼠进行行为学观察。结果:与空白对照组相比,20 mg.kg-1的黄芩苷、黄芩素、汉黄芩苷和汉黄芩素可分别将强迫游泳小鼠不动时间由(107.6±28.5)s缩短至(64.4±25.1),(73.2±30.8),(69.6±20.4),(79.7±32.6)s,并将悬尾不动时间由(85.2±27.3)s缩短至(43.1±16.4),(52.0±21.1),(48.3±25.9),(62.2±35.8)s,差异具有显著性意义,其中以黄芩苷和汉黄芩苷的作用尤为显著。另外,4种黄酮成分对小鼠的自发活动无明显影响。结论:4种黄酮成分均具有不同程度的抗抑郁活性,其中黄芩苷、汉黄芩苷的活性较强,这可能与这些药物在体内的代谢过程有关。
Objective: To screen the antidepressant activity of four major flavonoids in Astragalus membranaceus. METHODS: Male ICR mice were randomly divided into 10 groups: blank control group, positive control fluoxetine group (20 mg.kg-1), baicalin, baicalein, wogonin and wogonin. Four flavonoids were divided into 10 mg.kg-1 and 20 mg.kg-12 dose groups. Continuous ig administration 7 d. One hour after the last administration, mice were subjected to behavioral observations by forced swimming, tail suspension, and opening. RESULTS: Compared to the control group, baicalin, baicalein, wogonin and wogonin at 20 mg.kg-1 each reduced the immobility time of forced swimming mice from (107.6±28.5) s to (64.4). ±25.1), (73.2 ± 30.8), (69.6 ± 20.4), (79.7 ± 32.6) s, and shortening the tail suspension time from (85.2 ± 27.3) s to (43.1 ± 16.4), (52.0 ± 21.1) , (48.3 ± 25.9), (62.2 ± 35.8) s, the difference has significant significance, among which baicalin and crotonin have a particularly significant effect. In addition, the four flavonoids had no significant effect on the spontaneous activity of mice. Conclusion: The four flavonoids all have different degrees of antidepressant activity. Baicalin and wogonin have a strong activity, which may be related to the metabolism of these drugs in vivo.