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本实验观察了血细胞对血浆过氧化氢诱发化学发光值(H_2O_2-ICL)的影响。结果表明,正常兔血浆H_2O-2-ICL值明显高于全血和红细胞悬液,发生溶血后上述三组份的发光值均显著增加,其中全血和血浆的发光值分别增加15.5和6.1倍。油酸肺损伤兔所有组份的发光值均显著高于对照兔,同时其血浆H_2O_2-ICL发光值的衰变系数明显低于对照兔。以上结果提示:红细胞及其碎片显著影响血浆H_2O_2-ICL值,溶血促使其发光增强,而油酸肺损伤时血浆H_2O_2-ICL值的显著增加,则提示氧自由基代谢产物是其发光值增加的物质基础。
In this experiment, we observed the influence of blood cells on the hydrogen peroxide-induced chemiluminescence (H_2O_2-ICL) in plasma. The results showed that the plasma H_2O-2-ICL of normal rabbits was significantly higher than that of whole blood and erythrocyte suspensions. The luminescence values of these three components increased significantly after hemolysis, and the luminescence values of whole blood and plasma increased by 15.5 and 6.1 times. The luminescence values of all fractions of oleic acid-induced lung injury rabbits were significantly higher than that of the control rabbits, and the decay coefficient of plasma H 2 O 2-ICL luminescence was significantly lower than that of the control rabbits. The above results suggest that erythrocytes and their fragments significantly affect the plasma H_2O_2-ICL value, hemolysis to promote its luminescence, while the significant increase of plasma H_2O_2-ICL value during acute oleic acid-induced lung injury suggests that the oxygen free radical metabolites increase their luminescence values Material basis.