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目的:为揭示蛇足石杉Huperzia serrata居群间和居群内石杉碱甲(HupA)含量的变异程度和变异规律,以及引种前后的变化情况。方法:以采自浙江、广西和重庆的3个蛇足石杉居群,共73株活体植物为研究对象,用改进的HPLC测定了每株引种前后的HupA含量,采用变异系数、单因素方差分析和配对t检验等统计方法,分析了居群间和居群内HupA含量的多样性特征,以及引种前后的含量变化规律。结果:蛇足石杉中石杉碱甲含量在居群间和居群内存在极其丰富的多样性,居群间的含量差异达显著或极显著水平,居群内含量的变异系数从东到西分别为0.36,0.43,0.40;同园引种1年后,东部的浙江居群与西部的广西和重庆居群间的含量差异仍保持极显著的水平,而西部的广西和重庆居群间含量的显著性差异则消失,同时,各居群个体的石杉碱甲含量在引种前后均未发生明显的变化。结论:蛇足石杉中石杉碱甲含量的高低是遗传因素与环境条件共同作用的结果,但更受遗传因素控制。
Objective: To reveal the degree of variation and variation of HupA content in Huperzia serrata population and its population, as well as the changes before and after introduction. Methods: A total of 73 living plants were collected from three populations of Huperzia serrata from Zhejiang, Guangxi and Chongqing. The content of HupA in each plant before and after the introduction of each plant was determined by improved HPLC. The coefficient of variation, single-factor analysis of variance And paired t test were used to analyze the diversity of HupA content in populations and populations, and the variation of HupA content before and after introduction. Results: The content of huperzine A in Hupehuishenshanensis was extremely rich in inter-population and intra-population, and the difference was significant or extremely significant among the populations. The coefficient of variation of intra-population content varied from east to west 0.36, 0.43, 0.40. After 1 year of introduction of the same garden, the content difference between Zhejiang population in the east and Guangxi and Chongqing populations in western China still maintained a significant level, while the content in the populations of Guangxi and Chongqing in western China was significant Sexual differences disappeared, at the same time, each population of individual huperzine A content before and after introduction of no significant changes. Conclusion: The content of huperzine A in Hippeastrum snakeii is the result of the combination of genetic factors and environmental conditions, but it is controlled by genetic factors.