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对36个水稻骨干系进行了RFLP(限制性内切酶长度多态性)分析,并对这些水稻材料的162个杂交组合育性进行了分组分类研究。杂种育性的遗传基础研究表明,亚种内杂交组合育性显著高于亚种间杂交组合育性。亚种间杂交不育性是由籼粳两个亚种间深刻的遗传分化引起的。广亲和性品种为亲本之一的杂交组合多数(71.7%)具有正常的育性,RFLP分析表明,这些杂交组合的遗传基础特殊,其遗传距离大于典型的亚种内组合距离,而小于典型的亚种间遗传距离。广亲和性水稻种质的创建、改造和利用潜力是大的,广亲和基因作用及其与杂种优势表现有关的若干遗传育种学问题要协同一并去解决。
RFLP (restriction endonuclease length polymorphism) analysis was performed on 36 rice lines, and the 162 hybrid combinations of these rice materials were grouped and classified. The genetic basis of hybrid fertility studies have shown that within the subspecies cross hybrid fertility was significantly higher than the inter-subspecies hybrid fertility. Interspecific hybrid sterility is caused by the profound genetic differentiation between two subspecies of indica and japonica. The majority of hybrid combinations (71.7%) with one of the most compatible varieties had normal fertility. The RFLP analysis showed that the genetic basis of these hybrid combinations was special and the genetic distance was larger than the typical sub-species combination distance Less than the typical genetic distance between subspecies. The potential for creation, transformation and utilization of broad-compatible rice germplasm is large. The role of broad-compatibility genes and their genetic and breeding issues related to heterosis performance should be solved together.