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目的探讨5-氟尿嘧啶(5-Fu)联合柳氮磺胺吡啶(sulfasalazine)对细胞株BxPC-3抗增殖作用。方法噻唑蓝(MTT)比色法、流式细胞仪、相差显微镜分别检测不同浓度(12.5、25.0、50.0、100.0)mg/L5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3生长抑制率、细胞周期、凋亡率及细胞形态变化。结果不同浓度5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3生长抑制率呈时间、剂量依赖性;100mg/L5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3凋亡率从63%(12h)增加到93%(48h),与对照组比较[t=48.87(12h),263.15(48h),P<0.01];G0/G1期细胞从80%(12h)到97%(48h),与对照组比较[t=5.97(12h),10.39(48h),P<0.01];相差显微镜观察两者联合作用12h大量细胞固缩变圆,随时间延长胞体萎缩裂解。结论两者联合作用细胞株BxPC-3有协同抑制作用,与细胞周期、凋亡率及形态变化相关。
Objective To investigate the antiproliferative effects of 5-fluorouracil combined with sulfasalazine on BxPC-3 cell line. Methods MTT assay, flow cytometry and phase contrast microscope were used to detect the growth inhibition rates of BxPC-3 cells treated with different concentrations of (12.5,25.0,50.0,100.0) mg / L 5-Fu and 0.5 mmol / L sulfasalazine. Cell cycle, apoptosis rate and cell morphological changes. Results The growth inhibition rates of BxPC-3 cells treated with different concentrations of 5-Fu combined with 0.5mmol / L sulfasalazine were time-and dose-dependent. The apoptosis rate of BxPC-3 cells treated with 100mg / L 5-Fu combined with 0.5mmol / L sulfasalazine was 63% (48h), 263.15 (48h), P <0.01]. The cells in G0 / G1 phase changed from 80% (12h) to 97% (48h) Compared with the control group [t = 5.97 (12h), 10.39 (48h), P <0.01]; the phase contrast microscopy observed a large number of cells after 12h systolic rounding, cell atrophy lysis over time. Conclusion The combined effect of the two cell lines BxPC-3 synergistic inhibition, and cell cycle, apoptosis rate and morphological changes.