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用膜片钳技术对培养的纹状体神经元膜上的乙酰胆碱激活的离子通道进行了研究。结果表明，单通道电流为内向电流，随着超极化程度增大而增加，随着去极化程度增大而减少；翻转电位为１５．０±６．１ｍＶ，电导为８２．７１±１６．１７ｐＳ；通道的开放时间分布直方图和关闭时间分布直方图均需双指数拟合，开放时间分布直方图的时间常数分别为１．９３ｍｓ和５．７３ｍｓ，关闭时间分布直方图的时间常数则为０．５２８ｍｓ和２．３２ｍｓ；通道的平均开放时间和开放概率均与超极化程度无明显相关关系。由于电极液中含ＡＣｈ和Ｋ￣＋通道阻断剂Ｃｓ￣＋而不含Ｎａ￣＋或Ｃａ￣（２＋），且浴槽液中含Ｎａ￣＋通道阻断剂河豚毒素，因此可排除内向Ｎａ￣＋流、Ｃａ￣（２＋）流和Ｋ￣＋流，提示内向电流为ＡＣｈ激活的通道电流。
Acetylcholine-activated ion channels on cultured striatal neuronal membranes were studied using patch-clamp techniques. The results show that the single-channel current is an inward current, which increases with the increase of hyperpolarization and decreases with the depolarization degree. The flip-flop potential is 15.0 ± 6.1mV and the conductance is 82.71 ± 16 .17pS; channel open time histogram and close time histogram need double exponential fitting, the open time distribution histogram of the time constant were 1.93ms and 5.73ms, off time distribution histogram time constant then 0.528ms and 2.32ms respectively. The mean open time and open probability of the channels have no significant correlation with the degree of hyperpolarization. As the electrode solution containing ACh and K ~ + channel blocker Cs ~ + without Na ~ + or Ca ~ (2+), and the bath containing Na ~ + channel blocker tetrodotoxin, it can be excluded in Na ¯ + flow, Ca ~ (2+) flow and K ~ + flow, suggesting that the inward current is the ACh-activated channel current.