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目的:探讨喉癌患者血清中血管内皮生长因子C和D(vascular endothelial growth factor-C and-D,VEGF-Cand VEGF-D)在喉癌患者血清中的表达及其与肿瘤微血管(micro vessel density,MVD)的关系。方法:32例喉良性病变为对照组3,2例喉癌患者为实验组,应用ELISA法检测血清中VEGF-C、VEGF-D及Flt-1蛋白的表达,免疫组化SABC法检测喉组织中微血管的水平。结果:喉癌患者血清中组织VEGF-C、VEGF-D及Flt-1蛋白表达明显高于正常喉组织(P<0.01)。实验组患者血清VEGF-C和VEGF-D及Flt-1的表达与MVD成正相关有关(P<0.05)。结论:喉癌VEGF-C和VEGF-D及Flt-1与MVD密切相关,可作为评估喉癌患者预后的重要参考指标。
Objective: To investigate the expression of serum vascular endothelial growth factor-C and vascular endothelial growth factor-C and VEGF (D-VEGF-C and VEGF-D) in the serum of patients with laryngeal cancer and its relationship with the micro vessel density , MVD) relationship. Methods: Twenty-two patients with laryngeal benign lesions were selected as the control group and 3 patients with laryngeal cancer as the experimental group. The expressions of VEGF-C, VEGF-D and Flt-1 protein were detected by ELISA. The expression of VEGF- Microvascular level. Results: The expression of VEGF-C, VEGF-D and Flt-1 in serum of laryngeal cancer patients was significantly higher than that of normal laryngeal tissues (P <0.01). The serum levels of VEGF-C, VEGF-D and Flt-1 in the experimental group were positively correlated with MVD (P <0.05). Conclusions: The expressions of VEGF-C, VEGF-D and Flt-1 in laryngeal carcinoma are closely related to MVD, which can be used as an important reference index for assessing the prognosis of laryngeal cancer patients.