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目的 分析和预测牛带绦虫成虫烯醇酶基因及其编码蛋白的结构和功能,用于指导其生物学功能的实验研究。方法 利用生物信息学网站的各种在线分析工具,并结合其它大型生物信息学分析软件包,如Vector NTI suite,分析从牛带绦虫全长cDNA质粒文库中识别的烯醇酶基因的结构和编码区序列,并分析、预测编码的蛋白质的理化特性、抗原表位、翻译后的修饰、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。结果 该基因全长1 641 bp,编码区为133～1 096 bp,编码320个氨基酸,相对分子量理论预测值为34866.8 Mr。具有一从内到外的跨膜区,无各种亚细胞定位序列,具有多个潜在的磷酸化位点,蛋白的理化性质稳定。有13个主要的B细胞抗原表位。结论 通过生物信息学分析从牛带绦虫成虫cDNA质粒文库中发现烯醇酶基因,并预测得到其结构与功能方面的信息。
Objective To analyze and predict the structure and function of the enolase gene and its encoded protein of adult Taenia saginata as an experimental study to guide its biological function. Methods Using the various online analytical tools on the bioinformatics website and other large bioinformatics analysis software packages such as Vector NTI suite, the structure and coding of the enolase gene identified from the full-length cDNA plasmid library of Taenia saginata Region sequences, and analyze and predict the physicochemical properties, antigenic epitopes, post-translational modifications, functional domains, subcellular localization, topological structures, secondary structures, three-dimensional conformations and the like of the encoded proteins. Results The gene was 1 641 bp in length with a coding region of 133-1 096 bp encoding a protein of 320 amino acids with a theoretical predicted relative molecular weight of 34866.8. There is a transmembrane region from the inside to the outside, without various subcellular localization sequences, with a number of potential phosphorylation sites, the physical and chemical properties of protein stability. There are 13 major B cell epitopes. Conclusion The enolase gene was found in the cDNA plasmid library of adult Taenia sagittatum by bioinformatics analysis and its structure and function were predicted.