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目的:明确在乙、丁型肝炎病毒重叠感染时HDV对HBV复制和HBVM表达有无抑制作用。方法:HBV DNA定量采用聚合酶链反应法,抗HD IgM采用CIA抗体捕获法,抗HD采用EIA竞争法,HDAg采用EIA夹心法,HBVM采用酶联免疫吸附法。结果:重叠感染组和单纯乙肝组的HBV DNA定量高滴度(≥10~6cps/ml)病例数比率分别为75％和67.74％,各型肝炎(慢性肝炎、肝硬变、慢性重型肝炎)HBV DNA定量高滴度病例数比率,重叠感染分别为68.42％、71.43％、100％,单纯乙肝组分别为65.38%、66.67％、100％。两组病例HBVM主要表达模式均为HBsAg(+)、HBeAg(+)、抗HBc(+)或HBsAg(+)、抗HBe(+)、抗HBc(+),重叠感染组分别为57.14％、17.86％,单纯乙肝组分别为54.84％、16.13％,两组主要表达模式的HBV DNA定量高滴度病例数比半,重叠感染组分别为87.5％、60％,单纯乙肝组分别为88.24％、60％,均无显著性差异(P>0.05)。结论:HDV对HBV的复制和HBVM表达无明显抑制作用。
Objective: To determine whether HDV can inhibit HBV replication and HBVM expression in hepatitis B and D hepatitis viruses. Methods: HBV DNA was quantified by polymerase chain reaction, anti-HD IgM by CIA antibody capture, anti-HD by EIA competition, HDAg by EIA sandwich and HBVM by enzyme-linked immunosorbent assay. Results: The ratio of HBV DNA titer (≥10 ~ 6cps / ml) in overlapping infection group and simple hepatitis B group was 75% and 67.74%, respectively. All kinds of hepatitis (chronic hepatitis, cirrhosis and chronic severe hepatitis) The number of cases with high titer of HBV DNA titer and overlap infection were 68.42%, 71.43% and 100%, respectively, and those in pure hepatitis B group were 65.38%, 66.67% and 100% respectively. The main expression patterns of HBVM in both groups were 57.1% for HBsAg (+), HBeAg (+), anti HBc (+) or HBsAg (+), anti HBe (+) and anti HBc 17.86%, 54.84% and 16.13% respectively in the simple hepatitis B group. The number of high-titer and high-titer cases of HBV DNA in the two groups was 87.5% and 60% respectively, and 88.24% 60%, no significant difference (P> 0.05). Conclusion: HDV has no obvious inhibitory effect on HBV replication and HBVM expression.