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目的应用数目可变串联重复序列(variable number of tandem repeats,VNTRs)分析技术探讨绵阳地区结核分枝杆菌的基因分型,为结核病的防治提供科学依据。方法选取绵阳地区结核分枝杆菌临床分离菌株,采用聚合酶链反应(PCR)分别对VNTR位点进行检测分析,应用BioNumerics 5.0软件进行聚类分析。结果共对79株结核分枝杆菌的15个VNTR位点进行了检测,结果显示明显的基因多态性。各个位点的分辨能力不同,QUB-11b位点的分辨指数(Hunter-Gaston index,HGI)最高,为0.880;ETR C的HGI最低,为0.234;总HGI达到1。经聚类分析,79株菌可分为7个基因群、79个基因型,以Ⅲ群和Ⅴ群所占比例较大,Ⅲ群含37株菌,占46.8%;Ⅴ群含33株菌,占41.8%,其他菌株呈散在分布。结论绵阳地区结核分枝杆菌VNTRs基因存在明显的多态性,主要流行菌群为Ⅲ群和Ⅴ群,应加强此两群菌株的监控。
Objective To explore the genotyping of Mycobacterium tuberculosis in Mianyang by variable number of tandem repeats (VNTRs) analysis and to provide a scientific basis for the prevention and treatment of tuberculosis. Methods The clinical isolates of Mycobacterium tuberculosis in Mianyang were selected and the VNTR loci were detected by polymerase chain reaction (PCR). BioNumerics 5.0 software was used for cluster analysis. Results A total of 15 VNTR sites of 79 strains of Mycobacterium tuberculosis were detected. The results showed significant genetic polymorphisms. The resolution of each locus was different. The highest HGI of QUB-11b loci was 0.880. The lowest HGI of ETR C was 0.234 and the total HGI was 1. According to the cluster analysis, 79 strains were divided into 7 gene groups and 79 genotypes, accounting for a large proportion of group Ⅲ and group Ⅴ, 37 group of group Ⅲ comprising 46.8%, Ⅴ Group containing 33 strains of bacteria , Accounting for 41.8%, other strains were scattered. Conclusion There are obvious polymorphisms of Mycobacterium tuberculosis VNTRs gene in Mianyang region. The main epidemic groups are group Ⅲ and group Ⅴ. The surveillance of these two strains should be strengthened.