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采用Ｐｕｌｓｉｎｅｌｉ方法对兔脑缺血再灌注模型进行实验，用放免方法测定血管内皮素（ＥＴ）及血小板膜糖蛋白（ＧＭＰ１４０）变化。结果：缺血组实验前ＥＴ平均值为４７．１７ｎｇ／Ｌ，实验后１小时增高至９４．２０ｎｇ／Ｌ，１小时后呈持续性增高（１４９．３８～１４９．４２ｎｇ／Ｌ），与对照组比较有显著性差异（Ｐ分别＜０．０５和０．０１）；缺血组ＧＭＰ１４０实验前平均值为５５．６ｎｇ／Ｌ，实验后４小时增高至１３１．３ｎｇ／Ｌ以后逐渐下降，至２４小时时下降明显，与对照组比较亦有显著差异；ＥＴ与ＧＭＰ１４０的变化呈直线正相关（ｒ＝０．９９７６，Ｐ＜０．０１）。表明：ＥＴ的双峰变化与缺血再灌注时的血管内皮、神经组织受损有关；ＧＭＰ１４０的增高有一定的时间性。作者认为两者间的相互作用是通过血管内皮细胞血小板粒细胞的激活环路完成的。具体机制尚待探讨
The Pulsineli method was used to test the model of cerebral ischemia-reperfusion in rabbits. The changes of ET and GMP140 were determined by radioimmunoassay. Results: Before the experiment, the mean value of ET in the ischemic group was 47.17ng / L, increased to 94.20ng / L at 1 hour and increased continuously after 1 hour (149.38-149.42ng / L) (P <0.05 and 0.01, respectively). The mean of GMP140 before ischemia in experimental group was 55.6ng / L, then decreased to 131.3ng / L at 4h after the experiment, and then decreased gradually to There was a significant positive correlation between ET and GMP140 (r = 0.9976, P <0.01). The results showed that: the bimodal changes of ET were related to the damage of vascular endothelium and nerve tissue during ischemia-reperfusion; the increase of GMP140 had some timeliness. Authors believe that the interaction between the two is through the endothelial cell platelet granulocyte activation loop completed. The specific mechanism to be explored