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目的:研究黄芪注射液对原代培养大鼠海马神经元放射性损伤的保护作用,为放射性脑损伤的预防和治疗提供新的方法。方法:30Gy的X射线单次照射培养至12d的海马神经元,用DAPI染核方法检测海马神经元凋亡情况。结果:30Gy组在照射后24h核固缩百分数为(27.3±3.78)%,较0Gy组(1.88±1.03)%差异有统计学意义,P<0.01;30Gy+黄芪注射液0.5g/L预处理组在照射后24h核固缩百分数为(6.86±2.27)%,较30Gy组(P<0.01)及0Gy组(P<0.01)差异均有统计学意义。结论:应用黄芪注射液可以显著减少海马神经元放射性损伤导致的凋亡,提高其对放射性损伤的耐受能力。
OBJECTIVE: To study the protective effect of Astragalus injection on the radioactive injury of primary cultured hippocampal neurons in rats, and to provide a new method for the prevention and treatment of radiation-induced brain injury. Methods: The hippocampal neurons cultured to 30 days with 30 Gy of X-rays were irradiated with DAPI. The apoptosis of hippocampal neurons was detected by DAPI staining. Results: The nuclear pyknosis percentage of 30Gy group was (27.3 ± 3.78)% at 24 hours after irradiation, which was significantly lower than that of 0Gy group (1.88 ± 1.03)%, P <0.01; 30Gy + Astragalus injection 0.5g / L pretreatment group The nuclear pyknosis percentage at 24h after irradiation was (6.86 ± 2.27)%, which was significantly higher than that in 30Gy group (P <0.01) and 0Gy group (P <0.01). Conclusion: Astragalus injection can significantly reduce apoptosis induced by radioactive injury of hippocampal neurons, and improve their tolerance to radioactive injury.