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目的分析研究肺炎克雷伯菌对喹诺酮类及氨基糖苷类耐药基因的携带情况。方法从医院收治的患者标本中分离出46株肺炎克雷伯菌,使用VITEK-2型全自动细菌鉴定系统对细菌行进一步鉴定,采用纸片琼脂扩散法检测喹诺酮类和氨基糖苷类的耐药性,并分析耐药基因qnr A、qnr B、qnr S及aac(6)-Ib。结果 46株肺炎克雷伯菌中25株检测出aac(6)-Ib基因,占54.3%;14例检测出qnr S基因,占30.4%;8株检测出qnr B基因,占17.4%;qnr A的检出率为0;10株同时检测出氨基糖苷类和喹诺酮类耐药基因,占21.7%。结论 qnr基因在肺炎克雷伯菌中十分常见,而对氨基糖苷类产生耐药主要是因为受到aac(6)-Ib基因的影响。
Objective To analyze the carriage of quinolones and aminoglycoside resistance genes in Klebsiella pneumoniae. Methods 46 strains of Klebsiella pneumoniae were isolated from the specimens of patients admitted to the hospital. The bacteria were further identified by VITEK-2 automatic bacterial identification system. Quinolone and aminoglycoside resistance The resistance genes qnr A, qnr B, qnr S and aac (6) -Ib were analyzed. Results The aac (6) -Ib gene was detected in 25 out of 46 strains of Klebsiella pneumoniae, accounting for 54.3%. The qnr S gene was detected in 14 cases (30.4%), the qnr B gene was detected in 8 strains (17.4%), qnr The detection rate of A was 0; 10 strains simultaneously detected aminoglycoside and quinolone resistance genes, accounting for 21.7%. Conclusion The qnr gene is very common in Klebsiella pneumoniae, while the resistance to aminoglycosides is mainly due to the influence of aac (6) -Ib gene.