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目的探讨家蝇3龄幼虫血淋巴40%固相萃取组分抗肿瘤作用及机制。方法应用四甲基偶氮噻唑蓝(MTT)法检测40%固相萃取组分对S180肉瘤细胞株生长的抑制作用;通过体内试验检测40%固相萃取组分对S180小鼠实体瘤生长的抑制率,测定荷瘤小鼠血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量变化。结果在体外,40%固相萃取组分25μg/mL对S180肉瘤细胞增殖抑制率最高达79%,与阴性对照组比较差异有统计学意义(t=6.40,P<0.05);40%固相萃取组分有抑制S180实体瘤小鼠肿瘤生长的作用,高、中、低剂量组肿瘤生长抑制率分别为46.23%、35.23%、32.13%;与模型对照组(3.19±0.80)、(163.41±22.65)U/mL比较,3个剂量组的血清T-AOC和SOD活性均升高,其中高剂量组小鼠血清T-AOC和SOD活性分别为(4.91±0.46)、(213.19±43.37)U/mL,差异均有统计学意义(tT-AOC=7.12、tSOD=-7.16,P<0.01);3个剂量组的血清MDA含量均降低,其中高剂量组血清MDA含量为(8.85±1.81)nmol/mL,差异有统计学意义(tMDA=6.79,P<0.01)。结论 40%固相萃取组分在体外和体内均具有抑制S180肉瘤细胞生长的作用,清除自由基可能是其体内抗肿瘤机理。
Objective To investigate the anti-tumor effect and mechanism of 40% solid phase extraction of haemolymph in 3rd instar larvae of housefly. Methods The inhibitory effect of 40% SPE on the growth of S180 sarcoma cell line was detected by MTT assay. The effect of 40% solid phase extraction on the growth of S180 mouse solid tumor (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) in tumor-bearing mice. Results In vitro, 25μg / mL 40% SPE could inhibit the proliferation of S180 sarcoma cells by up to 79%, which was significantly different from that of the negative control group (t = 6.40, P <0.05) The extracted components could inhibit tumor growth in S180 solid tumor mice. The inhibition rates of tumor growth in high, medium and low dose groups were 46.23%, 35.23% and 32.13%, respectively. Compared with model control group (3.19 ± 0.80) and (163.41 ± 22.65) U / mL, serum T-AOC and SOD activities were increased in the three dose groups, and the serum T-AOC and SOD activities of high dose group were (4.91 ± 0.46), (213.19 ± 43.37) U / mL, the differences were statistically significant (tT-AOC = 7.12, tSOD = -7.16, P <0.01). The serum MDA levels were decreased in the three dose groups. The serum MDA level in the high dose group was (8.85 ± 1.81) nmol / mL, the difference was statistically significant (tMDA = 6.79, P <0.01). Conclusion 40% solid phase extraction can inhibit the growth of S180 sarcoma cells in vitro and in vivo, and scavenging free radicals may be its anti-tumor mechanism in vivo.