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目的通过常规饲料添加蛋黄和花生米的方法诱发建立雄性SD营养性肥胖大鼠动物模型。方法 30只4周龄SD雄性大鼠随机分为实验组24只,对照组6只。实验组饲喂正常饲料并添加鸡蛋黄及花生米,饲喂2周后实验组剔除肥胖抵抗大鼠,对照组饲喂正常饲料。大鼠饲喂8周后称量大鼠体质量并计算Lee’s指数,禁食12 h后处死,腹主动脉取血分离血清,检测血清中总胆固醇(TC)、甘油三脂(TG)含量,同时取肝脏组织进行组织病理学检查。结果实验组大鼠饲喂8周后,体质量达到526.6±46.4 g,对照组大鼠体质量为415.6±33.7 g,实验组大鼠体质量超过对照组体质量26.7%;实验组的Lee’s指数为330.0±8.2,对照组Lee’s指数为307.0±6.6(P<0.01),实验组与对照组比较有极显著差异。实验组的大鼠的血清TG均值为2.16±0.17 mmol/L、TC均值为1.40±0.34 mmol/L,对照组血清TG均值为1.66±0.11 mmol/L,TC均值为0.82±0.13 mmol/L,两组大鼠比较有极显著性差异。病理学结果显示:实验组大鼠肝脏均出现脂肪样变。结论 4周龄SD雄性大鼠采用正常饲料并添加鸡蛋黄及花生米的方法能稳定成功地建立营养性肥胖大鼠模型。
OBJECTIVE To establish animal model of male SD nutritional obesity by means of routine feeding of egg yolk and peanut. Methods Thirty male SD rats of 4 weeks old were randomly divided into experimental group (24 rats) and control group (6 rats). The experimental group was fed with normal diet and egg yolk and peanut were added. After two weeks of feeding, the obese rats were eliminated from the experimental group, while the control group was fed with the normal diet. After 8 weeks of feeding, the rats were weighed and the Lee’s index was calculated. After fasting for 12 hours, the rats were sacrificed, the blood was collected from the abdominal aorta to separate the serum, the levels of total cholesterol (TC), triglyceride (TG) At the same time take the liver tissue for histopathological examination. Results The body weight of the rats in the experimental group was 526.6 ± 46.4 g after 8 weeks of feeding, the body weight of the control rats was 415.6 ± 33.7 g, the body weight of the rats in the experimental group was 26.7% of the control group. The Lee’s index Was 330.0 ± 8.2, and Lee’s index of the control group was 307.0 ± 6.6 (P <0.01). There was a significant difference between the experimental group and the control group. The mean serum TG of the experimental group was 2.16 ± 0.17 mmol / L, the mean of TC was 1.40 ± 0.34 mmol / L, the mean serum TG of the control group was 1.66 ± 0.11 mmol / L, the mean of TC was 0.82 ± 0.13 mmol / L, Two groups of rats have very significant difference. Pathological results show that: the experimental group of rats showed fatty degeneration in the liver. Conclusion 4-week-old male Sprague-Dawley rats can be used successfully to establish a nutritious obese rat model by using normal diet and adding egg yolk and peanut.