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[Objectives] To conduct molecular cloning and characteristic analysis of Farnesyl Pyrophosphate Synthase(FPS) so as to provide the foundation for molecular regulation of steroid alkaloid biosynthesis in Fritillaria thunbergii.[Methods]A pair of degenerate primers were designed based on the conservative sequences of the cloned FPS gene from other plant species,and this study used RT-PCR and Ta cloning technology as well as q PCR to analyze the bioinformation of FPS and the expression of FPS in different parts of Fritillaria thunbergii. [Results] A 414 bp gene was gained,and sequence analysis showed that the conserved sequence fragments of Fritillaria thunbergii FPS gene had high identity(93%- 76%) to other plants,and the deduced amino acid sequence was predicted to have high homology with other plants(96%-82%) and was closely related to Lilium longiflorum. Gene expression analysis revealed that the expression of FPS was highest in flower,and was followed by bulb and stem.[Conclusions]FPS gene of Fritillaria thunbergii was initially determined and named Ft FPS as the first reported FPS gene from Fritillaria thunbergii,laying a foundation for effective utilization of the gene.
[Objectives] To conduct molecular cloning and characteristic analysis of Farnesyl Pyrophosphate Synthase (FPS) so as to provide the foundation for molecular regulation of steroid alkaloid biosynthesis in Fritillaria thunbergii. [Methods] A pair of degenerate primers were designed based on the conservative sequences of the cloned FPS gene from other plant species, and this study used RT-PCR and Ta cloning technology as well as q PCR to analyze the bioinformation of FPS and the expression of FPS in different parts of Fritillaria thunbergii. [Results] A 414 bp gene was gained, and sequence analysis showed that the conserved sequence fragments of Fritillaria thunbergii FPS gene had high identity (93% - 76%) to other plants, and the deduced amino acid sequence was predicted to have high homology with other plants (96% 82%) and was closely related to Lilium longiflorum. Gene expression analysis revealed that the expression of FPS was highest in flower, and was followed by bulb and stem. [Conclusions] FP S gene of Fritillaria thunbergii was found determined and named Ft FPS as the first reported FPS gene from Fritillaria thunbergii, laying a foundation for effective utilization of the gene.