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Nakai(1979)在液氮(-196℃)条件下处理水稻种子,发现在相同突变频率下,能减轻 M_1代的生理损伤和染色体畸变。高明尉等(1982)将水稻种子在γ射线照射前,进行液氮冷冻处理,则发现会加剧二叶期秧苗的损伤。鉴于液氮对生物的作用及其机制的研究还刚刚开始,很多现象不清,机理不明,特别是与辐射诱变结合研究更少。为此,我们在研究辐射对蚕豆染色体畸变的基础上,进一步探索在液氮下辐射的染色体畸变效应。
Nakai (1979) treated rice seeds under liquid nitrogen (-196 ℃) and found that the same mutation frequency can reduce the physiological damage and chromosomal aberration of M_1 generation. Gao Ming Wei et al (1982) before the γ-ray irradiation of rice seeds for liquid nitrogen cryogenic treatment, it was found to aggravate the two-leaf stage seedling damage. In view of the role of liquid nitrogen on biological and its mechanism has just begun, many phenomena are unclear, the mechanism is unknown, especially with the combination of radiation mutagenesis less. Therefore, based on the study of the chromosome aberration caused by radiation on Vicia faba, we further explored the chromosomal aberration effect of radiation under liquid nitrogen.