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目的比较新城疫病毒(NDV)强毒株D817、弱毒株7793和La Sota疫苗株对人喉癌Hep-2细胞的杀伤效应。方法3株病毒经扩增、噬斑纯化后,分别感染Hep-2细胞和喉正常组织细胞,一定时间后,显微镜下观察细胞形态变化,MTT比色法检测病毒对细胞的杀伤效应。结果3株病毒均能在Hep-2细胞中增殖并杀伤细胞,杀伤效应以D817株最高,La Sota株其次,7793株最低,且差异均无统计学意义。杀伤效应与病毒剂量和作用时间呈正相关,显微镜下可见明显的细胞病变。而对喉正常组织细胞的杀伤效应不明显。结论NDV D817株和7793株对喉癌细胞的杀伤效应与La Sota疫苗株相似,而对喉正常组织细胞的杀伤效应不明显,有望成为喉癌生物学治疗新的候选病毒株。
Objective To compare the killing effects of Newcastle disease virus (NDV) virulent strain D817, attenuated strain 7793 and La Sota vaccine against human laryngeal carcinoma Hep-2 cells. Methods Hep-2 cells and laryngeal normal tissue cells were infected by three strains of viruses after amplification and plaque purification. The cell morphology was observed under a microscope after a certain period of time. The killing effect of the virus on the cells was detected by MTT assay. Results All three viruses could proliferate and kill cells in Hep-2 cells. The killing effect was highest in D817 strain, followed by La Sota strain and 7793 strain in the latter. There was no significant difference between the three strains. The killing effect was positively correlated with the dose and duration of the virus, and obvious cytopathic effect was observed under the microscope. The laryngeal normal tissue cells killing effect is not obvious. Conclusion The killing effect of NDV D817 strain and 7793 strain on laryngeal cancer cells is similar to that of La Sota vaccine strain, but their effect on normal laryngeal squamous cell is not obvious. It is expected to be a new candidate strain for biological treatment of laryngeal cancer.