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目的:研究丹参酮ⅡA(TanⅡA)对缺血-再灌注(I/R)大鼠神经元的保护作用及其机制。方法:线栓法建立局灶性大脑中动脉闭塞(MCAO)再灌注模型,选取造模成功动物64只SD雄性大鼠随机分为8组,分别为正常对照组、假手术组、I/R模型24 h与72 h组、I/R+4 mg/kg TanⅡA治疗24 h与72 h组、I/R+8 mg/kg TanⅡA治疗24 h与72 h组,TanⅡA治疗后观察各组脑组织病理学变化,免疫组化法检测脑组织Caspase-3蛋白的表达水平及TUNEL法检测脑组织凋亡细胞的数量。结果:(1)8 mg/mL TanⅡA治疗可改善I/R模型大鼠脑组织形态病理变化,减少神经细胞凋亡和坏死;(2)TanⅡA治疗组Caspase-3阳性细胞数均有下调,其中以I/R 72 h+8 mg/mL TanⅡA组最为显著(P<0.05);(3)TanⅡA8 mg/mL治疗组可见凋亡细胞减少(P<0.05)。结论:TanⅡA可显著减轻I/R大鼠的脑组织损伤,减少Caspase-3蛋白的表达及减少细胞凋亡,进而延缓、减轻神经元在I/R损伤后的凋亡和坏死,TanⅡA对脑I/R损伤大鼠有明显的神经保护作用。
Objective: To study the protective effect of TanⅡA on neurons in ischemia-reperfusion (I / R) rats and its mechanism. Methods: A focal reperfusion model of middle cerebral artery occlusion (MCAO) was established by thread occlusion. Sixty SD male rats were randomly divided into 8 groups: normal control group, sham operation group, I / R The rats in 24 h and 72 h groups were treated with TanⅡA at I / R + 4 mg / kg for 24 h and 72 h, and TanⅡA at I / R + 8 mg / kg for 24 h and 72 h. Pathological changes, immunohistochemistry to detect the expression of Caspase-3 protein in brain tissue and TUNEL method to detect the number of apoptotic cells in brain tissue. Results: (1) Treatment with TanⅡA at 8 mg / mL could improve morphological and pathological changes of brain in I / R model rats and reduce apoptosis and necrosis of nerve cells. (2) The number of Caspase-3 positive cells in TanⅡA treatment group was decreased (P <0.05). (3) TanⅡA 8 mg / mL treatment group showed reduced apoptosis cells (P <0.05). Conclusion: TanⅡA can significantly reduce the damage of brain tissue, decrease the expression of Caspase-3 protein and decrease the apoptosis in I / R rats, and then delay and reduce the apoptosis and necrosis of neurons after I / R injury. I / R injury rats have obvious neuroprotective effect.