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[目的]探讨p16INK4A在新柏氏宫颈液基细胞学(TCT)标本中各级宫颈上皮内瘤变的表达,评价其在宫颈病变中表达的意义。[方法]采用Envision法,检测76例TCT标本及相应宫颈活检组织中p16INK4A表达。[结果]在TCT中,p16INK4A在无上皮内病变(NILM)、非典型鳞状细胞(ASC)、低级别鳞状上皮内病变(LSIL)、高级别鳞状上皮内病变(HSIL)阳性表达率分别为0、15.78%、45.45%、88%。在相应活检组织中,p16INK4A在宫颈炎、CINⅠ、CINⅡ、CINⅢ中阳性表达率分别为0、63.15%、89.28%、100%。p16INK4A在TCT和相应活检组织中表达的符合率为91.23%。[结论]TCT标本的p16INK4A表达检测可以提高宫颈高级别病变的检出率。
[Objective] To investigate the expression of p16INK4A in cervical intraepithelial neoplasia at various stages in neo-Burke’s cervical liquid-based cytology (TCT) and to evaluate its significance in cervical lesions. [Methods] Envision method was used to detect the expression of p16INK4A in 76 cases of TCT specimens and corresponding cervical biopsy tissues. [Results] The positive rate of p16INK4A in non-epithelial lesions (NILM), atypical squamous cells (ASC), low grade squamous intraepithelial lesion (LSIL) and high grade squamous intraepithelial lesion (HSIL) Respectively 0,15.78%, 45.45%, 88%. In the corresponding biopsies, the positive rate of p16INK4A in cervicitis, CINⅠ, CINⅡ and CINⅢ were 0,63.15%, 89.28% and 100% respectively. The coincidence rate of p16INK4A expression in TCT and corresponding biopsy was 91.23%. [Conclusion] The detection of p16INK4A expression in TCT specimens can improve the detection rate of high grade cervical lesions.