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目的建立毛细管区带电泳法测定人体尿样中双氯灭痛、氯丙嗪含量的方法。方法采用弹性石英毛细管(31.5cm,25μmi.d.,360μmo.d.),以4.90×10-3mol/LNa2HPO4-7.80×10-3mol/LNaH2PO4(pH=7)为缓冲液,10kV分离电压,5kV电渗进样10s,碳纤维电极工作电极(长200μm,直径8μm),检测电势0.72V。结果双氯灭痛、氯丙嗪两种药物分别在9.90×10-6～5.00×10-4mol/L(r=0.9982)、5.0×10-7～1.0×10-4mol/L(r=0.9998)范围内表现出良好的线性,其回收率分别为104%,96.0%。结论该方法简便、快速、准确,可作为人体尿样中双氯灭痛、氯丙嗪的分离检测方法。
Objective To establish a method for the determination of diclofenac and chlorpromazine in human urine by capillary zone electrophoresis. Methods The elastic quartz capillary (31.5cm, 25μmi.d., 360μmo.d.) Was used in this experiment. The buffer solution was 4.90 × 10-3mol / LNa2HPO4-7.80 × 10-3mol / LNaH2PO4 (pH = 7) Electroosmotic injection 10s, carbon fiber electrode working electrode (length 200μm, diameter 8μm), detection potential 0.72V. Results Both diclofenac and chlorpromazine were in the range of 9.90 × 10-6 ~ 5.00 × 10-4mol / L (r = 0.9982) and 5.0 × 10-7 ~ 1.0 × 10-4mol / L (r = 0.9998) Showed a good linearity, the recovery rates were 104%, 96.0%. Conclusion The method is simple, rapid and accurate and can be used as a method for the separation and detection of diclofenac and chlorpromazine in human urine samples.