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发展较低成本、自动化规模化生产组培小植株的繁殖体系，必将在农业和森林中变得越来越重要。过去一直认为组培小植株几乎没有光合能力。因此，在培养基中必须加入糖来提供碳源，以保证小植株异养的需要。然而，最近发现，如培养瓶中ＣＯ２浓度、光照环境因素等被适当控制，组培小植株仍然具有光合能力，可以发展自养。在传统的密闭培养瓶中，光期的ＣＯ２浓度常低于１００（１０－６）且在大多数情况下小植株不能发展正碳平衡。据实验，在无糖的培养基中，在一个高光合电子流下富集ＣＯ２可以促进组培小植株生长，且几乎没有细菌或真菌污染。因此，可使用大培养瓶以降低劳动力消耗，并采用自动化大规模生产系统。
The development of lower cost and automation of large-scale production of tissue culture plantlets breeding system will inevitably become more and more important in agriculture and forests. In the past it has been assumed that tissue culture plantlets have little photosynthetic capacity. Therefore, sugars must be added to the medium to provide a source of carbon to assure the heterotrophic needs of the plantlets. However, it has recently been found that, for example, CO2 concentrations in culture flasks, light environmental factors and the like are properly controlled, and plantlet tissue plants still have photosynthetic ability to develop autotrophs. In conventional closed culture flasks, the CO2 concentration at photoperiod is often less than 100 (10-6) and in most cases the plantlet can not develop a positive carbon balance. It has been experimentally demonstrated that CO2 enrichment in a sugar-free medium under a high photoelectron flux can promote the growth of tissue culture plantlets with little or no bacterial or fungal contamination. As a result, large flasks can be used to reduce labor costs and to use automated mass production systems.