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目的 探讨电刺激面肌有无预防废用性肌萎缩的作用。方法 制作实验性面神经麻痹模型 ,电刺激眼轮匝肌 (简称眼肌 ) 3周后 ,测试眼肌肌纤维径、神经纤维径、再生神经纤维数 ,扫描电镜观察眼肌次级突触间隙面积。结果 电剌激和非电刺激侧眼肌细胞横断面积分别为 (4 6 6 .7±2 8.2 ) μm2 和 (4 78.2± 13 .6 ) μm2 ,差异无显著性。眼肌支配神经纤维数 ,电刺激侧平均 (10 6 .2± 8.1)根 ,非剌激侧平均 (187.6± 1.2 )根 ,显著小于非刺激侧 (t=2 .2 36 ,P <0 .0 5 )。电刺激侧神经纤维横断面积平均 (5 .32± 0 .30 ) μm2 ,非刺激侧平均 (9.6 6± 0 .90 ) μm2 ,非常显著小于非刺激侧 (t=2 .96 9,P <0 .0 1)。电刺激侧眼肌次级突触间隙面积比非刺激侧明显减小。结论 电刺激面肌无预防肌萎缩作用 ,反而抑制了面神经纤维再生。
Objective To investigate the effect of electro-stimulated facial muscle on the prevention of disuse muscle atrophy. Methods Experimental facial paralysis model was made. After 3 weeks of orbital muscle stimulation (referred to as ocular muscle), the muscle fiber diameter, nerve fiber diameter and number of regenerated nerve fibers were measured, and the subocclusal synaptic gap area was observed by scanning electron microscopy. Results There was no significant difference in the number of ocular muscle cells between electro-stimulated and non-stimulated groups (466.7 ± 8.22 μm2 and (4.78.2 ± 13.6) μm2, respectively. The number of intraocular nerve fibers, the average of electrical stimulation side (106.2 ± 8.1) and the non-stimulation side (187.6 ± 1.2) were significantly smaller than those of non-stimulation side (t = 2.26, P <0. 0 5). The average area of lateral nerve fibers (5.32 ± 0.30) μm2 and non-stimulated side (9.6 6 ± 0.90) μm2 were significantly smaller than those on the non-stimulated side (t = 2.96 9, P <0 .0 1). The area of secondary synaptic cleft in the side of electrical stimulation was significantly smaller than that in the non-stimulated side. Conclusion Electrical stimulation of facial muscle without preventing muscle atrophy, but inhibited facial nerve fiber regeneration.