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目的对饥饿小鼠和饱食小鼠分别注射地塞米松和肾上腺激素,观察激素对其肝糖原及血糖含量的影响。方法 32只健康小鼠随机分成两组:饥饿组16只,严格禁食不禁水30 h,于实验前5 h对其中8只小鼠腹腔注射地塞米松;饱食组16只,自由摄食、饮水,于实验前0.5 h对其中8只小鼠腹腔注射肾上腺素。采用蒽酮显色法测定小鼠肝糖原,用葡萄糖氧化酶法测定小鼠血糖。结果饥饿组注射地塞米松小鼠与未注射小鼠血糖、肝糖原含量差异显著,饱食组小鼠注射肾上腺素与未注射小鼠血糖、肝糖原含量亦差异显著,均有统计学意义(P<0.05)。结论肾上腺素能增加血糖含量、降低肝糖原含量,而地塞米松能使血糖含量下降,肝糖原含量上升。
Objective To study the effects of hormones on glycogen content and blood glucose in starvation mice and satiety mice. Methods Thirty-two healthy mice were randomly divided into two groups: 16 in the starvation group, severe fasting for 30 h, dexamethasone in 8 mice 5 h before the experiment, 16 in the fed group, Drinking water, intraperitoneal injection of epinephrine into eight of them at 0.5 h before the experiment. The liver glycogen of mice was determined by anthrone colorimetric method and the blood glucose of mice was measured by glucose oxidase method. Results In the starvation group, the blood glucose and the content of glycogen in dexamethasone-injected mice and non-injected mice were significantly different. The levels of blood sugar and hepatic glycogen were also significantly different between the starvation group and the non-injected mice Significance (P <0.05). Conclusion Adrenergic can increase the content of blood sugar, reduce the content of hepatic glycogen, while dexamethasone can make the blood sugar content decreased, the content of hepatic glycogen increased.