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本研究用人群实验，探讨了寒冷条件下，高脂膳食和维生素Ｅ对人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性和血浆脂质过氧化的影响，分析了酶活性与血浆ＭＤＡ间的关系。结果表明：摄入３５％（产能量比）高脂膳食１５天后，人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性显著高于１２％膳食脂肪组（Ｐ＜０．０５），血浆ＭＤＡ含量比实验前略有降低。高脂膳食同时每天补充２００ｍｇ维生素Ｅ组，Ｎａ，Ｋ一ＡＴＰａｓｅ活性比单纯高脂膳食组明显升高（Ｐ＜０．０５），而血浆ＭＤＡ含量则比实验前显著降低（Ｐ＜０．０５）。相关分析表明，各组实验前后酶活性与ＭＤＡ含量问均呈负相关。由此可见，高脂膳食和ＶＥ均能使人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性升高；ＶＥ可降低血浆中ＭＤＡ含量。ＭＤＡ与Ｎａ，Ｋ一ＡＴＰａｓｅ呈负相关。推测可能是脂肪和ＶＥ通过保护细胞膜引起了酶活性升高。
In this study, we investigated the effects of high-fat diet and vitamin E on Na, K-ATPase activity and plasma lipid peroxidation in human erythrocytes under cold conditions, and analyzed the relationship between enzyme activity and plasma MDA. The results showed that the Na, K-ATPase activity of human erythrocyte membrane was significantly higher than that of 12% dietary fat group (P <0.05) after 35 days high-fat diet with 35% reduce. Compared with the high-fat diet group, the activities of Na, K-ATPase in the high-fat diet group and the vitamin E group supplemented with 200mg / day were significantly increased (P <0.05) ). Correlation analysis showed that there was a negative correlation between enzyme activity and MDA content before and after each experiment. Thus, high-fat diet and VE can make human erythrocyte membrane Na, K-ATPase activity increased; VE can reduce plasma MDA content. MDA and Na, K-ATPase was negatively correlated. It is speculated that fat and VE may cause increased enzyme activity by protecting the cell membrane.