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目的:探讨地塞米松对油酸诱导大鼠急性呼吸窘迫综合征(ARDS)后肺纤维化的影响。方法:将54只SPF级雄性SD大鼠随机分为3组(n=18)。A组注射生理盐水作为正常对照;B、C组以油酸(0.15 ml/kg)制作模型,制模后6 h测动脉血气,以氧合指数≤200为模型成功,A、B组经静脉注射生理盐水(5 ml/kg),c组经静脉给予地塞米松(2 mg/kg),每天1次,连续1w。各组在给药后第7 d、14 d、28 d麻醉后处死大鼠,测定支气管肺泡灌洗液(BALF)中Ⅲ型前胶原(PcⅢ)浓度,留取右侧肺经包埋后行Masson染色。结果:B组和C组PcⅢ浓度在第7 d、14 d、28 d时均明显高于A组(P<0.05),且C组明显低于B组(P<0.01),C组肺纤维组织增殖较B组明显减轻。结论:地塞米松可能对ARDS后肺纤维化有抑制作用。
Objective: To investigate the effect of dexamethasone on pulmonary fibrosis in rats with oleic acid-induced acute respiratory distress syndrome (ARDS). Methods: Fifty-four SPF male SD rats were randomly divided into three groups (n = 18). Rats in group A and group C were injected normal saline. Group B and C were given oleic acid (0.15 ml / kg), and arterial blood gas was measured at 6 h after modeling. The oxygenation index was less than or equal to 200, Normal saline (5 ml / kg) was injected. Group c was given dexamethasone (2 mg / kg) once a day for 1 week. Rats in each group were sacrificed on the 7th, 14th and 28th day after the administration, and the concentration of type Ⅲ procollagen (Ⅲ) in bronchoalveolar lavage fluid (BALF) was determined. Masson staining. Results: The PcⅢ concentrations in group B and group C were significantly higher than those in group A on day 7, 14 and 28 (P <0.05), and were significantly lower in group C than those on group B (P <0.01) Tissue proliferation significantly reduced compared with the B group. Conclusion: Dexamethasone may inhibit pulmonary fibrosis after ARDS.