盐胁迫下SOD模拟物对水稻幼苗生物量及SOD活性的影响

• 作 者: 林庆斌 丁杨 王海波 张昭其 毛小云 乐学义
• 机 构: 华南农业大学理学院,华南农业大学园艺学院,华南农业大学资源与环境学院,
• 出 处: 土壤通报
• 发表日期: 2009年05期
• 关键词: 模拟物 SOD SOD模拟物 幼苗生物量 水稻幼苗 盐胁迫 水稻浸种 盐害 喷施 光还原

在中度盐害的情况下,用四种SOD模拟物[Cu(phen)(L-ala)(H2O)]Cl·4H2O,[Cu(phen)(L-ser)(H2O)]Cl,[Cu(phen)(L-glu)(H2O)]Cl,[Cu(phen)(L-tyr)(H2O)]Cl·2H2O)(L-ala,L-ser,L-glu和L-tyr分别为L-丙氨酸,L-丝氨酸,L-谷氨酸及L-酪氨酸)对水稻浸种、叶面喷施。三叶期时,测定了其生物量并采用NBT光还原法测定了配合物对水稻幼苗SOD的影响。实验结果表明,经模拟物处理后,水稻幼苗的生物量提高了3.70～40.74%(鲜重)和2.29～37.67%(干重),体内SOD活性提高最大达42.31%。表明这些模拟物能有效缓解水稻幼苗所受的盐害胁迫。

In moderate salt damage, four SOD mimics [Cu (phen) (L-ala) (H2O)] Cl · 4H2O, [Cu (phen) (L-ser) Cu (phen) (L-glu) (H2O)] Cl, [Cu (phen) (L-tyr) (H2O)] Cl2.2H2O (L-ala, L-ser, L-glu and L-tyr L-alanine, L-serine, L-glutamic acid and L-tyrosine) on rice soaking, foliar spraying. At the three-leaf stage, the biomass was measured and the effect of the complex on the SOD of rice seedlings was determined by NBT photoreduction. The results showed that the biomass of rice seedlings increased by 3.70 ~ 40.74% (fresh weight) and 2.29 ~ 37.67% (dry weight) by the simulant treatment, and the SOD activity increased by 42.31% in vivo. These results indicate that these mimics can effectively alleviate the salt stress of rice seedlings.