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目的 探讨小檗碱对家兔颈动脉粥样硬化形成的干预作用。方法 2 4只日本大耳白家兔随机分成正常组、对照组和小檗碱干预组,正常组8只,普通饲料喂养5周;对照组8只,高脂喂养一周后行颈总动脉内膜空气干燥术,术后继续喂养4周;小檗碱干预组8只,高脂喂养一周后行颈总动脉内膜空气干燥术,术后继续高脂喂养并每日肌肉注射小檗碱,剂量为5 mg/ kg,时间为4周。实验满5周后,行右侧脑血管造影(DSA)术并观察正常组、对照组和小檗碱干预组的颈动脉的病理变化和计算内膜中膜比(I/ M)。结果 正常组的颈动脉的内膜围一层单皮细胞,中膜为规则的平滑肌肌层;对照组内膜明显增厚,中膜变薄,HE染色可见大量泡沫细胞堆积和坏死的物质;小檗碱干预组内膜也有增厚的现象,增厚程度明显小于对照组,HE染色见有泡沫细胞形成,堆积程度小于对照组。DSA发现对照组的颈动脉有不同程度的狭窄,而小檗碱预防组的家兔没有发现明显的颈动脉狭窄。结论 小檗碱可以干预家兔颈动脉粥样硬化的形成过程,延缓粥样硬化的发生。
Objective To investigate the effect of berberine on carotid atherosclerosis in rabbits. Methods 2 4 Japanese white rabbits were randomly divided into normal group, control group and berberine intervention group, normal group 8, normal feed 5 weeks; control group 8, high fat diet for one week after the common carotid artery The rats in the berberine group were given the air-drying of the common carotid artery one week after the high-fat diet. The mice were continuously fed with high fat diet and intramuscularly injected with berberine after operation. The dose was 5 mg / kg for a period of 4 weeks. Five weeks after the experiment, the right cerebral angiography (DSA) was performed and the carotid artery pathological changes and I / M ratio of the normal group, the control group and the berberine intervention group were observed. Results In the normal group, the carotid intima surrounded by a layer of monolayers, the middle of the membrane is a regular smooth muscular layer; the control group was significantly thicker intima, the media thinning, HE staining shows a large number of foam cell accumulation and necrosis of the material; Berberine intervention group also thickening of the endometrial phenomenon, the degree of thickening was significantly less than the control group, HE staining foam cells formed, the accumulation of less than the control group. The DSA found that the carotid arteries of the control group had varying degrees of stenosis, whereas no significant carotid stenosis was found in the rabbits in the berberine prevention group. Conclusion Berberine can interfere with the formation of carotid atherosclerosis in rabbits and delay the occurrence of atherosclerosis.