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在实验室家猫感染周期型马来丝虫较为困难。我们采用将感染期幼虫直接注射到腘窝淋巴结的方法感染家猫成功,并对微丝蚴的周期性进行了观察,结果报告如下。一、材料与方法 (一)感染期幼虫的收集:用本所实验室饲养驯化的中华按蚊,光照下以胎盘膜饲血瓶喂食37℃混有周期型马来丝虫感染长爪沙鼠腹腔液的兔血。饲血后于28℃饲养11天,贝氏分离法收集感染期幼虫,备用。 (二)实验动物及感染方法实验用6只家猫均购自马来丝虫非流行区。猫龄6月～4岁,人工感染前血检均未发现微丝蚴。实验感染时,将家猫固定于实验台上,轻度麻醉。
Domestic cats infected with periodic malayan filariasis are more difficult in laboratory. We used the infection larvae injected directly into the popliteal lymph node infection in cats success, and periodicity of microfilaria were observed, the results are reported as follows. First, the material and methods (A) the collection of larvae during infection: Domesticated Anopheles sinensis in our laboratory, fed with placental membrane blood bottles at 37 ℃ mixed with periodic Malay worm infected gerbils Peritoneal fluid of rabbit blood. Feeding at 28 ℃ after feeding for 11 days, Bayesian isolation method collection of larvae, spare. (B) Experimental Animals and Infections Methods Six domestic cats were purchased from the non-endemic area of Malayan filariasis. Cat age 6 months to 4 years old, blood tests were not found before artificial microfilaria. Experimental infection, the domestic cat fixed on the experimental stage, mild anesthesia.