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目的研究冠心病(CHD)与甘露聚糖结合凝集素(MBL)基因多态性及其血浆MBL和高敏C-反应蛋白(hs-CRP)水平的关系。方法收集100例CHD患者(CHD组)和60例健康人群(对照组)抗凝血,限制片段长度多态性法(PCR-RFLP)检测MBL ExonI 54位密码子基因点突变,ELISA法和免疫比浊法分别检测血浆MBL含量和高敏C-反应蛋白(hs-CRP)水平。结果 CHD组与对照组MBL基因ExonI 54位密码子点突变频率比较差异无统计学意义(P>0.05)。CHD组血浆MBL和hs-CRP含量均高于对照组(P<0.01)。而CHD组内血浆hs-CRP水平升高者(>3 mg/L)与hs-CRP水平正常者(<3 mg/L)MBL含量比较差异无统计学意义(P>0.05)。结论 MBL和hs-CRP可能以不同机制参与CHD发生、发展过程。
Objective To investigate the relationship between CHD and mannan-binding lectin (MBL) gene polymorphism and plasma MBL and high-sensitivity C-reactive protein (hs-CRP) levels. Methods The anticoagulant and restriction fragment length polymorphism (PCR-RFLP) methods were used to detect point mutations in codon 54 of MBL Exon I gene in 100 CHD patients (CHD group) and 60 healthy people (control group) Nephelometry was used to detect plasma MBL levels and high-sensitivity C-reactive protein (hs-CRP) levels respectively. Results There was no significant difference in mutation frequency of Exon 54 codon between CHD group and control group (P> 0.05). The levels of MBL and hs-CRP in CHD group were higher than those in control group (P <0.01). However, there was no significant difference in MBL content of plasma hs-CRP level (> 3 mg / L) and normal hs-CRP level (<3 mg / L) in CHD group (P> 0.05). Conclusion MBL and hs-CRP may participate in the occurrence and development of CHD by different mechanisms.