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【目的】探讨 Src酪氨酸激酶抑制剂Ⅱ对膀胱移行细胞癌T24细胞 Src蛋白下游信号钙黏附蛋白 E(E-Cadherin)的作用。【方法】应用半定量 RT-PCR法检测0μmol/L、0.2μmol/L、1.0μmol/L、5.0μmol/L Src 酪氨酸激酶抑制剂Ⅱ处理后的人膀胱癌T24细胞各浓度组的 E-Cadherin表达情况。【结果】Src 酪氨酸激酶抑制剂Ⅱ处理后,E-Cadherin表达呈上调趋势；人膀胱癌T24细胞E-Cadherin mRNA表达水平随加入Src Kinase Inhibitor II浓度增加呈上调趋势,0.2μmol/L、1.0μmol/L、5.0μmol/L浓度组OD比值显著高于0μmol/L浓度组,组间比较差异均具有统计学意义(P <0.05)。【结论】Src酪氨酸激酶抑制剂Ⅱ对 c-Src蛋白下游信号 E-Cadherin具有上调作用,进一步证实了 c-Src蛋白及其下游信号 E-Cadherin作为膀胱移行细胞癌分子靶向治疗的可能性。“,”Obj ective]To investigate the function of Src Kinase Inhibitor Ⅱ on the downstream signal of E-Catherin of bladder transitional cell carcinoma T24 cells Src protein.[Methods]We examined the level of E-Cad-herin in T24 cell lines as well as the effect of Src Kinase InhibitorⅡwith 0μmol/L、0.2μmol/L、1.0μmol/L、5.0μmol/L on Src expression,activated by semi-quantitive RT-PCR.[Results]After the treatment of Src Kinase in-hibitor Ⅱ,the expression of E-Cadherin was up-regulated;expression levels of human bladder cancer T24 cells E-Cadherin mRNA were up-regulated with Src Kinase Inhibitor Ⅱ concentration increased,OD ratio of 0.2 mol/L,1 mol/L,5 mol/L concentration group was significantly higher than that of 0 mol/L concentration group,the differ-ence was statistically significant (P <0.05).[Conclusion]Src Kinase inhibitor Ⅱ can up-regulated c-Src protein downstream signal E-Cadherin,which further confirmed that the treatment possibility of c-Src protein and the downstream signal of E-Cadherin can play the role as a molecular target in bladder transitional cell carcinoma .