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自Brunner等介绍以~(51)Cr标记靶细胞的同位素释放法测定杀伤T细胞(CTL)的细胞毒作用以来,~(51)Cr释放法已成为测定CTL杀伤能力的常规。但~(51)Cr标记靶细胞的自发释放(SR)率高(1～2%/小时),不适用于较长培育时间的细胞毒试验。近年来~(111)In-oxine标记靶细胞被介绍来代替~(51)Cr标记,其优点为SR低(0.25
Since the release of ~ (51) Cr labeled target cells by Brunner et al. Described the cytotoxicity of cytotoxic T lymphocytes (CTLs), the release of ~ (51) Cr has become a routine measure of cytotoxicity against CTLs. However, the rate of spontaneous release (SR) of ~ (51) Cr-labeled target cells is high (1-2% / h) and is not suitable for cytotoxicity in longer incubation time. In recent years, ~ (111) In-oxine labeled target cells have been introduced instead of the ~ (51) Cr labeling, which has the advantages of low SR (0.25