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采用HeLa细胞入侵及其阻断试验 ,观察抗志贺菌菌膜蛋白和LPS的单抗对志贺菌入侵HeLa细胞的影响。并用免疫印迹法初步分析了感染细胞及其培养上清中的蛋白成分。结果抗菌膜蛋白的单抗 2 6C3不但没有阻断反而促进了细菌的入侵 ,在HeLa细胞胞浆内出现成堆的F2a菌 ,其绝对菌数远远超过未经该单抗处理的F2a菌感染的HeLa细胞组。免疫印迹显示单抗处理组细胞培养液中IpaB、IpaC蛋白明显比未经单抗处理组多。抗LPS的单抗对细菌入侵具有一定的阻断作用。提示抗菌膜蛋白单抗所识别的IpaB表位 ,具有调节F2a菌进入HeLa细胞的能力。其作用机制可能是通过细菌分泌器 ,使分泌IpaB、IpaC蛋白增多。
HeLa cells were used to invade and block test to observe the effect of anti-Shigella membrane protein and LPS monoclonal antibody on the invasion of Shigella to HeLa cells. Western blot analysis of the infected cells and culture supernatant of the protein composition. Results Antibacterial membrane protein mAb 2 6C3 not only failed to block but instead promoted the invasion of bacteria. In the cytoplasm of HeLa cells, there appeared piles of F2a bacteria whose absolute number of bacteria was much higher than that of F2a bacteria without the monoclonal antibody HeLa cell group. Immunoblotting showed that the IpaB and IpaC proteins in the cell culture medium of the monoclonal antibody treatment group were significantly higher than those in the group without mAb treatment. Anti-LPS monoclonal antibody has certain blocking effect on bacterial invasion. Prompted antibacterial membrane monoclonal antibody identified IpaB epitopes, with regulating F2a bacteria into HeLa cells. Its mechanism of action may be through the bacterial secretor, secretion of IpaB, IpaC protein increased.