对节白蜡皮中秦皮甲素和秦皮乙素的含量测定方法

• 作 者: 马力 严慧娟 曲佳佳 张玉
• 机 构: 华中科技大学同济医学院附属协和医院药剂科,华中科技大学同济医学院药学院,
• 出 处: 中国医院药学杂志
• 发表日期: 2010年15期
• 关键词: 秦皮乙素 对节白蜡 含量测定 高效液相色谱法 甲醇提取 标准曲线方程 供试品溶液 对照品溶液 液相色谱图 环烯醚萜

目的:建立对节白蜡皮中秦皮甲素和秦皮乙素的含量测定方法。方法:采用甲醇提取样品。固定相为HypersilC18柱(150mm×4.6mm,5μm),流动相:乙腈-水(25∶75),流速1mL.min-1,紫外检测波长336nm。结果:标准曲线方程为秦皮甲素A=2049.2C-142.5(r=0.9998),秦皮乙素A=961.75C-25.9(r=0.9997),线性范围都是2～10mg.L-1,平均回收率秦皮甲素为98.84%,秦皮乙素为99.72%。RSD秦皮甲素为1.83%,秦皮乙素为1.77%。结论:用HPLC方法测定对节白蜡皮中秦皮甲素和秦皮乙素的含量,简便、快速、准确。

Objective: To establish a method for the determination of aesculin and aesculetin in Pterocarya falciparum. Method: Samples were extracted with methanol. The stationary phase was a Hypersil C18 column (150 mm×4.6 mm, 5 μm), mobile phase: acetonitrile-water (25:75), flow rate 1 mL.min-1, UV detection wavelength 336 nm. RESULTS: The standard curve equation was apiosporin A = 2049.2C-142.5 (r = 0.9998), apigenin A = 961.75C-25.9 (r = 0.9997), and the linear range was 2 ~ 10 mg.L-1. The rate of apigenin was 98.84%, and apigenin was 99.72%. RSD aesculin was 1.83%, and apigenin was 1.77%. Conclusion: The HPLC method was used to determine the content of aesculin and aesculin in the paraffin wax, which was simple, rapid and accurate.