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为探讨原发性肾病综合征（ＩＮＳ）患儿淋巴细胞免疫功能的动态变化，分别采用噻唑蓝（ＭＴＴ）比色等方法，对２７例ＩＮＳ活动期患儿和其中１５例缓解期患儿的外周血淋巴细胞（ＰＢＬ）的增殖应答效应、白细胞介素２（ＩＬ－２）及其相应受体的表达能力和白细胞介素６（ＩＬ－６）的产生水平进行了检测。结果表明，ＩＮＳ活动期患儿ＰＢＬ的增殖应答能力比对照组和缓解组减低，并与Ｔ淋巴细胞亚群ＣＤ４／ＣＤ８比值呈正相关；活化的淋巴细胞ＩＬ－２受体表达和ＩＬ－２产生能力低下；而血浆和细胞培养上清液中可溶性ＩＬ－２受体水平升高。同时淋巴细胞培养上清液中ＩＬ－６含量增高。结果提示，ＩＮＳ患儿细胞免疫功能减低，影响体液免疫，激发其他细胞因子的产生，参与ＩＮＳ免疫病理过程的发生、发展。
To investigate the dynamic changes of lymphocyte immune function in children with primary nephrotic syndrome (INS), 27 cases of INS active stage and 15 cases of remission stage were treated with MTT assay The proliferation response of peripheral blood lymphocytes (PBLs), the expression of interleukin 2 (IL-2) and its receptor and the production of interleukin 6 (IL-6) were detected. The results showed that the proliferative and responsive ability of PBL in INS-active children was lower than that in control and remission groups and positively correlated with the ratio of CD4 / CD8 in T-lymphocyte subsets; the expression of IL-2 receptor in activated lymphocytes and IL-2 production Low capacity; and plasma and cell culture supernatant soluble IL-2 receptor levels. At the same time, the IL-6 content in lymphocyte culture supernatant increased. The results suggest that INS children with reduced cellular immune function, affecting humoral immunity, stimulate the production of other cytokines involved in the pathogenesis of INS immunopathology, development.