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本实验旨在通过输注脐血Lin-细胞 ,利用新生大鼠建立人 /大鼠造血嵌合体模型。将脐血来源的Lin-细胞移植到正常一级新生SD大鼠的肝脏内 ,移植后的新生大鼠与同窝未接种的正常新生大鼠共同饲养和观察 ,于输注Lin-细胞后 2 ,4和 8周时取嵌合鼠的外周血、脾脏等组织 ,检测人源细胞的比例及人特异性 β2 微球蛋白基因片段。结果发现 ,通过流式细胞术检测到嵌合鼠外周血中存在有一定比例的人源细胞 ,PCR证明了嵌合鼠脾脏基因组DNA中存在人特异性 β2 微球蛋白基因片段。结论 :利用新生大鼠可以建立人造血嵌合体 ,它为今后利用此实验模型进行人造血干细胞体内生物学特性的检测及其它实验奠定了基础 ,具有潜在应用价值
The purpose of this experiment is to establish a human / rat hematopoietic chimerism model using neonatal rats by infusing cord blood Lin-cells. The umbilical cord blood-derived Lin- cells were transplanted into the liver of normal first-degree newborn SD rats. The newborn rats after transplantation were co-housed and observed with normal neonatal rats without litter inoculation. After infusion of Lin- At 4 and 8 weeks, peripheral blood, spleen and other tissues of the chimeric mice were harvested to detect the proportion of human-derived cells and human-specific β2 microglobulin gene fragments. As a result, it was found that a certain percentage of human-derived cells were present in peripheral blood of chimeric mice by flow cytometry. PCR confirmed the presence of a human-specific β2 microglobulin gene fragment in the genomic DNA of the chimeric mouse spleen. Conclusion: The newborn rat can be used to establish artificial chimera, which laid the foundation for the future use of this experimental model for the detection of biological characteristics of human hematopoietic stem cells and other experiments, with potential application value