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目的研究山西地区汉族人群红细胞MNS、Kell两种稀有血型系统基因多态性分布特点,为人类群体遗传学、临床用血和自身输血及山西地区稀有血型库的建立奠定基础。方法本研究采用序列特异性引物-聚合酶链式反应(SSP-PCR)法,对150名山西地区汉族人群两种稀有血型系统进行基因分型,并进行统计学分析。结果山西地区汉族人群MNS、Kell两种稀有血型基因频率依次M=0.5533,N=0.4467,S=0.0367,s=0.9633,K1=0.0167,K2=0.9833,χ2检验比较基因型分布的观察值与期望值发现,检测结果符合Hardy-Weinberg平衡法则。结论山西地区汉族人群MNS稀有血型系统抗原基因频率分布呈多态性,而Kell稀有血型系统抗原基因频率分布呈单态性,且基因频率基本符合Hardy-Weinberg遗传平衡法则。
Objective To study the distribution of genetic polymorphisms of RNS and Kell erythrocytes in Shanxi Han population and lay a foundation for the establishment of genetic population, clinical use of blood and blood transfusion and establishment of rare blood bank in Shanxi Province. Methods In this study, we genotyped two rare blood group systems in 150 Han Chinese population in Shanxi Province using sequence-specific primer-polymerase chain reaction (SSP-PCR). Results The genotype frequency of MNS and Kell in Shanxi Han population were M = 0.5533, N = 0.4467, S = 0.0367, s = 0.9633, K1 = 0.0167 and K2 = 0.9833, respectively. The χ2 test compared the observed and expected genotype distribution Found that the test results in line with the Hardy-Weinberg equilibrium law. Conclusion The frequency distribution of MNS genotypes in Han population in Shanxi Province is polymorphic, while the frequency distribution of Kell rare blood type system antigen genes is single, and the gene frequency basically conforms to the Hardy-Weinberg genetic balance rule.