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分别用He-Ne激光(5.23 mW/mm2,5 min/d)和紫外-B辐射(UV-B,280~320 nm)(强度12μW/cm2,8 h/d)及二者复合处理红梗牛皮菜(Beta vulgarisvar.cicila),然后通过水解比色和高效液相色谱(HPLC)法分析叶中甲醇水溶性黄酮类化合物,并测定形态与生长指标。结果表明,UV-B辐射处理后黄酮类化合物含量增加到对照的160%,但激光复合处理后,则恢复到略高于对照的水平。其中槲皮素和黄酮在各处理组中变化非常显著,槲皮素在UV-B(B)、He-Ne激光(L)及二者复合(BL)处理后被大量诱导;而黄酮仅在对照组(CK)和L组出现。分析表明,He-Ne激光与UV-B辐射一样,对黄酮类化合物的诱导是具有选择性的,具有高UV吸收并具较高抗氧化作用的化合物更容易被诱导。因此He-Ne激光很可能通过这种选择机制来减轻UV-B导致的伤害。
The red stems were treated with He-Ne laser (5.23 mW/mm2, 5 min/d) and ultraviolet-B radiation (UV-B, 280-320 nm) (intensity 12μW/cm2, 8 h/d) and both. In the case of Bulgar (Beta vulgarisvar.cicila), the methanol-soluble flavonoids in leaves were analyzed by hydrolysis colorimetry and high performance liquid chromatography (HPLC) and the morphological and growth parameters were determined. The results showed that the content of flavonoids after UV-B radiation treatment increased to 160% of the control, but after laser treatment, it recovered to a level slightly higher than the control. Quercetin and flavonoids were significantly changed in each treatment group. Quercetin was significantly induced after UV-B (B), He-Ne laser (L), and both (BL) treatment; The control group (CK) and L group appeared. The analysis showed that the He-Ne laser, like UV-B radiation, is selective to the induction of flavonoids. Compounds with high UV absorption and high antioxidant activity are more likely to be induced. Therefore, He-Ne lasers are likely to reduce UV-B-induced damage through this selection mechanism.