Establishment of an orthotopic pancreatic cancer mouse model: Cells suspended and injected in Matrig

• 作 者: Yong-Jian Jiang Chong-Lek Lee
• 机 构: DepartmentofPancreaticSurgeryHuashanHospitalFudanUniversity,DepartmentofPathologyHuashanHospitalFuda
• 出 处: 世界胃肠病学杂志：英文版（电子版）
• 发表日期: 2014年28期
• 关键词: PANCREATIC cancer ORTHOTOPIC MOUSE model MATRIGEL Pancreatic cancer Orthotopic m
• 基金项目: The Shanghai Municipal Natural Science Foundation,No.11ZR1405500,the Shanghai Municipal Science and Technology Commission grant,No.13140902401

AIM:To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma.METHODS:Pan02 cells were suspended in low-temperature Matrigel and injected into the parenchyma of pancreatic tails