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转染外源野生型ｐ５３基因对膀胱癌ＥＪ细胞的抑制作用。方法将含外源野生型ｐ５３基因的（ａｄ┐Ｗｔｐ５３）转染膀胱癌ＥＪ细胞，观察生长曲线，细胞周期变化及裸鼠体内抑瘤试验。结果转染了ａｄ┐Ｗｔｐ５３的ＥＪ细胞在体外生长速率下降，在裸鼠体内致瘤性丧失，流式细胞仪显示，ＤＮＡ合成前期或静止期的细胞比例增高。另外，ａｄ┐Ｗｔｐ５３直接注射到肿瘤组织内，可使外源野生型ｐ５３基因在肿瘤细胞内有效、稳定的表达，肿瘤表现为生长减缓，最后停止生长并有缩小。结论腺病毒载体介导基因转染效率高，速度快，安全，是很有前途的体内基因治疗载体。
Transfection of exogenous wild type p53 gene inhibits bladder cancer EJ cells. Methods Human adriamycin-resistant bladder cancer cell line EJ (ad┐Wtp53) transfected with exogenous wild-type p53 gene was transfected to observe the growth curve, cell cycle and tumor inhibition in nude mice. Results EJ cells transfected with ad┐Wtp53 had a decreased growth rate in vitro and no tumorigenicity in nude mice. Flow cytometry showed that the proportion of cells in pre-or post-DNA synthesis increased. In addition, Ad ┐ Wtp53 directly injected into the tumor tissue can make foreign wild-type p53 gene expression in tumor cells effectively and stably, the tumor showed slow growth, and finally stop growing and shrinking. Conclusion Adenovirus vector-mediated gene transfection efficiency, fast, safe, is a promising vector for gene therapy in vivo.