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目的:分析SLE和RA患者外周血CD4+ CD25high Treg和CD4+ CD25low T细胞的数量变化以及两群细胞表面协同刺激分子PD-1分子的表达情况,初步探讨PD-1表达异常在SLE和RA患者细胞免疫失调中的意义。方法:流式细胞术(FCM)检测CD4+ CD25high Treg和CD4+ CD25low T细胞的比率以及PD-1的表达率。结果:与健康对照组相比,SLE和RA患者组外周血CD4+ CD25high Treg的比率均明显降低(P<0.05);两疾病组间相比,SLE组CD4+ CD25high Treg的比率更低(P<0.05)。RA患者组CD4+ CD25high Treg表面PD-1的表达率明显低于健康对照和SLE组(P<0.05),而SLE组该指标与健康对照无统计学差异(P>0.05)。CD4+ CD25low T细胞以及该群细胞上PD-1的表达率在三组间无统计学差异。结论:外周血CD4+ CD25high Treg生成不足导致其对效应性T细胞的抑制能力减弱是SLE和RA患者的共同特征;PD-1表达降低可能是RA患者CD4+ CD25high Treg免疫调节紊乱的重要机制,而在SLE患者PD-1分子并不是影响免疫稳态的主要因素。
OBJECTIVE: To analyze the changes of the numbers of CD4 + CD25high Treg and CD4 + CD25low T cells in peripheral blood of patients with SLE and RA and the expression of PD-1 on two cell surface co-stimulatory molecules, and to explore the cellular immunity of PD-1 in patients with SLE and RA The meaning of the imbalance. Methods: The ratio of CD4 + CD25high Treg and CD4 + CD25low T cells and the expression of PD-1 were detected by flow cytometry (FCM). Results: Compared with healthy controls, the percentage of CD4 + CD25high Tregs in peripheral blood was significantly lower in SLE and RA patients (P <0.05). The ratio of CD4 + CD25high Treg in SLE patients was lower than that in healthy controls (P <0.05) ). The expression of PD-1 on CD4 + CD25high Treg surface in RA patients was significantly lower than that in healthy controls and SLE patients (P <0.05). However, there was no significant difference between SLE patients and healthy controls (P> 0.05). There was no significant difference in the expression of PD-1 between CD4 + CD25low T cells and the group of cells in the three groups. CONCLUSION: Inadequate production of CD4 + CD25high Treg in peripheral blood leads to a decrease in inhibitory effect on effector T cells. It is a common feature of patients with SLE and RA. Decreased PD-1 expression may be an important mechanism of immune dysregulation of CD4 + CD25high Treg in RA patients. PD-1 molecules in SLE patients are not the main factors affecting immune homeostasis.