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目的 :探讨端粒酶活性在儿童急性白血病中的表达及临床意义。方法 :采用端粒重复系列扩增法(TRAP)检测了 11例儿童急性白血病化疗前、完全缓解后骨髓单个核细胞 (MNCs)端粒酶活性水平 ,对部分病例进行追踪观察 ,并与 10例正常儿童骨髓 MNCs端粒酶活性相比较。结果 :正常骨髓 MNCs具低水平的端粒酶活性 ,相对端粒酶活性范围 2 .6 %～ 19.1% ;急性白血病患儿化疗前相对端粒酶活性明显增高 40 .4%～ 98% ,完全缓解后其相对端粒酶活性为 2 1.7%～ 6 0 .1% ,随缓解时间的延长 ,端粒酶活性逐渐降低。结论 :端粒酶激活在儿童急性白血病的发生中可能具有重要作用 ,动态观察端粒酶活性水平 ,对估测病情、监测微小残留病可能具有实际意义
Objective: To investigate the expression of telomerase activity in children with acute leukemia and its clinical significance. Methods: The levels of telomerase activity in bone marrow mononuclear cells (MNCs) from 11 children with acute leukemia before chemotherapy were detected by TRAP (amplification of telomeric repeat) method. Some cases were followed up and compared with 10 Comparison of telomerase activity in normal children with bone marrow MNCs. Results: The normal bone marrow MNCs had low telomerase activity and relative telomerase activity ranged from 2.6% to 19.1%. The relative telomerase activity in children with acute leukemia was significantly increased by 40.4% to 98% before chemotherapy After treatment, the relative telomerase activity was 21.7% ~ 60.1%, and telomerase activity decreased gradually with the prolongation of remission time. CONCLUSION: Telomerase activation may play an important role in the pathogenesis of childhood acute leukemia. The dynamic observation of telomerase activity may be of practical significance in the assessment of disease and monitoring of minimal residual disease