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在正常人淋巴细胞,加入鼻咽癌低分化细胞(CNE-2)培养上清(CNE-2S)400μl/ml。分别测定不加入和加入CNE-2S的淋巴细胞内cAMP、IL-2及对IL-2的增殖活性,11例正常人淋巴细胞内cAMP含量平均值为13.77±4.92pm/1×10~6,加入CNE-2S后为26.1±15.14pm/l×10~6。比未加入前有明显升高(P<0.05)。9例正常人的IL-2活性的平均值为2048±1590cpm,加入后为1236±761cpm。比未加入前有明显降低(P<0.05)。9例正常人淋巴细胞对IL-2增殖活性平均值为4478±5755cpm,加入后降为2942±2706cpm,但统计学上无显著性(P>0.05)。结果表明,CNE-2S可提高正常人淋巴细胞内cAMP水平。与此同时,IL-2活性受到明显抑制,但淋巴细胞对IL-2增殖活性的变化不显著。
In normal human lymphocytes, nasopharyngeal carcinoma differentiated cells (CNE-2) culture supernatant (CNE-2S) 400 μl / ml was added. The activity of cAMP, IL-2 and IL-2 in lymphocytes without addition of CNE-2S and addition of CNE-2S were measured respectively. The average value of cAMP in 11 normal human lymphocytes was 13.77 ± 4.92 pm / 1 × 10 -6, 26.1 ± 15.14 pm / l × 10 -6 after addition of CNE-2S. Than before without significant increase (P <0.05). The mean IL-2 activity of 9 normal subjects was 2048 ± 1590 cpm, and 1236 ± 761 cpm after the addition. Than before without significantly reduced (P <0.05). Nine normal lymphocyte proliferation activity of IL-2 average 4478 ± 5755cpm, after adding to 2942 ± 2706cpm, but no statistically significant (P> 0.05). The results show that CNE-2S can increase cAMP levels in normal human lymphocytes. At the same time, IL-2 activity was significantly inhibited, but lymphocyte proliferation activity of IL-2 was not significantly changed.