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背景:如何选择中药治疗脑血管病,并且具有既提高脑血流量,又不影响血压和心率的作用,成了极有前途的研究方向。目的:观察养阴通脑颗粒对麻醉犬平均血压和心率变化的作用。设计:以杂种犬为研究对象,完全随机分组设计,随机对照实验。单位:解放军第四军医大学西京医院麻醉科及解放军军第四军医大学基础部药理科。材料:实验于2003-03/06在解放军第四军医大学生理教研室心血管实验室完成。实验选用健康杂种犬23只,雌雄不拘。将犬随机分为4组:养阴通脑颗粒大剂量(n=8),中剂量(n=6)和小剂量组(n=5)及生理盐水组(n=4)。方法:模仿人类口服中药方式分别给予养阴通脑颗粒大、中、小剂量组麻醉犬养阴通脑颗粒2.0,1.0,0.5g/kg。所有用药组按犬体质量计算药量,溶于100mL生理盐水中,通过胃管灌入胃内,生理盐水组用等量生理盐水灌胃。主动脉平均血压由股动脉插管经晶体压力传感器测量,心率从标准II导联心电图的R-R间期测算。记录用药前、用药后0.5,1.0,1.5,2.0,3.0,4.0,5.0,6.0h的平均血压和心率值。主要观察指标:各组犬用药前及用药后不同时间点血压和心率的变化。结果:纳入结果分析犬29只。血压变化:养阴通脑颗粒大、中剂量组平均血压降低,与用药前比较,最大降幅分别为-5.4%和-6.2%,小剂量组的血压有升有降,以降为主,升压的最大变化率为6.6%(P>0.05),降压最大变化率为-4.1%(P>0.05)。生理盐水组的平均血压最大变化率为-9.6%(P>0.05)。心率变化:大、中剂量组的心率随时间延长呈现减慢趋势,与用药前比较,大、中、小剂量组心率减慢的最大变化率分别为-4.4%,-12.2%和-9.5%,变化率数据差异无明显意义(P>0.05)。生理盐水组的心率随时间延长而逐渐减慢。结论:养阴通脑颗粒对麻醉犬平均血压和心率无明显的影响。
Background: How to choose Chinese medicine to treat cerebrovascular disease and have the effect of increasing cerebral blood flow without affecting blood pressure and heart rate has become a very promising research direction. Objective: To observe the effect of Yangyin Tongnao Granule on the change of average blood pressure and heart rate in anesthetized dogs. Design: Mongrel dogs as the research object, completely random grouping design, randomized controlled trials. Unit: Department of Pharmacology, Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Chinese PLA, and Fourth Military Medical University, PLA. MATERIALS: The experiment was performed at the Cardiovascular Laboratory of Department of Physiology, Fourth Military Medical University of PLA from March 2003 to June 2006. Twenty-three healthy mongrel dogs were selected for the experiment, either male or female. The dogs were randomly divided into 4 groups: large-dose of Yangyin Tongnao Granule (n=8), medium-dose (n=6) and low-dose group (n=5), and saline group (n=4). Methods: Mimic of human oral administration of traditional Chinese medicine respectively to Yangyintongnao Granules Large, medium and small dose group anesthetic dog Yangyintongnao particles 2.0, 1.0, 0.5g/kg. All medication groups were dosed according to dog body mass, dissolved in 100 mL normal saline, and injected into the stomach through the stomach tube. The normal saline group was intragastrically administered with the same volume of normal saline. The mean aortic blood pressure was measured by a femoral artery catheter through a crystal pressure transducer, and the heart rate was calculated from the R-R interval of a standard II lead electrocardiogram. Mean blood pressure and heart rate values before and 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 5.0, and 6.0 hours after administration were recorded. MAIN OUTCOME MEASURES: Changes in blood pressure and heart rate at different time points before and after treatment in each group of dogs. Results: 29 dogs were included in the analysis of the results. Blood pressure changes: The average blood pressure of large and medium-dose groups in Yangyintongnao Granules decreased, compared with pre-dosage, the largest decreases were -5.4% and -6.2%, respectively. The blood pressure in the low-dose group increased or decreased, mainly decreased, and boosted. The maximum change rate was 6.6% (P>0.05), and the maximum change rate of blood pressure was -4.1% (P>0.05). The mean change rate of mean blood pressure in the saline group was -9.6% (P>0.05). Changes in heart rate: Heart rate of the large and middle dose groups showed a trend of slowing over time. Compared with pretreatment, the maximum rates of heart rate decrease in large, medium, and low dose groups were -4.4%, -12.2%, and -9.5%, respectively. There was no significant difference in the rate of change (P>0.05). The heart rate of the saline group gradually decreased over time. Conclusion: Yangyintongnao granule has no obvious effect on average blood pressure and heart rate of anesthetized dogs.