Detailed Deletion Mapping of Chromosome 9p21-22 in Nasopharyngeal Carcinoma

• 作 者: 阳剑波 张晓梅 邓龙文 谭国林 周鸣 曾朝阳 曹莉 沈守荣 李桂源
• 出 处: Chinese Journal of Cancer Research
• 发表日期: 2000年03期
• 关键词: Mapping Carcinoma Chromosome microsatellite deletion chromosome heterozygosity a

Objective: To further refine the extent of deletion on chromosome 9p21-22 in nasopharyngeal carcinoma (NPC) and provide evidence for discovering new tumor suppressor gene. Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and tumor samples by using 11 high-density microsatellite polymorphic markers. Results: 17 of 25 cases (68.0%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion might be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21.1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.

Methods: Loss of heterozygosity (LOH) on chromosome 9p21-22 was analyzed in 25 paired blood and Higher frequencies of LOH were found at four loci: D9S161 (35.0%), D9S1678 (31.5%), ), D9S263 (33.3%) and D9S1853 (33.3%), where 6 cases had a contiguous stretch of allelic loss. Conclusion: The minimal common region of deletion likely be defined between D9S161 and D9S1853 (estimated about 2.7 cM in extent) at 9p21 .1, suggesting that inactivation of one or more tumor suppressor genes located in this region may be an important step in NPC.