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目的研究姜黄素对纳米二氧化硅(nm-SiO2)诱导人永生化表皮(HaCaT)细胞氧化损伤相关指标的改变及保护作用。方法通过CCK-8法检测HaCaT细胞在15 nm SiO2和姜黄素作用下的细胞活力水平,测定在姜黄素干预下HaCaT细胞内活性氧簇(ROS)水平和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的酶活力水平。结果姜黄素的干预能明显改善15 nm SiO2处理引起的HaCaT细胞的氧化损伤。与SiO2组相比,姜黄素干预组能有效提高细胞存活率、降低细胞内ROS的积累;另外,姜黄素干预可以降低两个氧化应激相关的酶SOD和CAT的酶活力水平。结论姜黄素干预对由于nm-SiO2引起的HaCaT细胞的氧化应激损伤具有一定的保护作用。
Objective To study the changes and protective effects of curcumin on the oxidative damage induced by nano-silica (nm-SiO2) in human immortalized epidermis (HaCaT) cells. Methods The cell viability of HaCaT cells under the action of 15 nm SiO2 and curcumin was detected by CCK-8 assay. The levels of reactive oxygen species (ROS) and superoxide dismutase (SOD) in HaCaT cells were determined by curcumin intervention Enzyme activity levels of catalase (CAT). Results The intervention of curcumin significantly improved the oxidative damage of HaCaT cells induced by 15 nm SiO2 treatment. Compared with SiO2 group, curcumin intervention group can effectively improve the cell survival rate, reduce the intracellular ROS accumulation; In addition, curcumin intervention can reduce the two oxidative stress-related enzymes SOD and CAT enzyme activity levels. Conclusion Curcumin can protect oxidative stress injury induced by nm-SiO2 in HaCaT cells.