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目的:采用HPLC-DAD法测定“武当二号金银花”叶中芦丁和金丝桃苷的含量.方法: 色谱柱为Fortis Xi Phenyl柱(250 mm×4. 6 mm,5 μm),流动相为乙腈-0. 5%冰醋酸水溶液,梯度洗脱,流速为0. 9 ml·min-1,检测波长为354 nm,柱温为30℃.结果:芦丁及金丝桃苷分别在9. 00~90. 00 μg·ml-1、16. 35~163. 50 μg·ml-1浓度范围内与峰面积呈良好的线性关系 (r=0. 999 5),平均回收率分别为99. 70% (RSD=1. 96% )、99. 30% (RSD=1. 95% ).结论: 该法简便、准确、重现性好、专属性强,可用于“武当二号金银花”叶中芦丁和金丝桃苷的含量测定.“,”Objective: To establish an HPLC-DAD method for the determination of rutin and hyperoside in the leaves of “WudangⅡ Flos Lonicerae”. Methods: A Fortis Xi Phenyl column (250 mm×4. 6 mm, 5 μm) was adopted, the mobile phase was acetoni-trile-0. 5% glacial acetic acid aqueous solution with gradient elution at a flow rate of 0. 9 ml·min-1, the detection wavelength was 354 nm, and the column temperature was 30℃. Results: The linear range of rutin (r=0. 999 5) and hyperoside (r=0. 999 5) was 9. 00-90. 00μg·ml-1and 16. 35-163. 50 μg·ml-1, and the average recovery was 99. 70% ( RSD =1. 96% ) and 99. 30% ( RSD =1. 95% ), respectively. Conclusion: The method is simple, accurate, reproducible and specific, which can be used for the determina-tion of rutin and hyperoside in the leaves of “Wudang Ⅱ Flos Lonicerae”.