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目的 观察血性脑脊液 (BCSF)对内皮细胞分泌内皮素 (ET)和一氧化氮 (NO)的影响及川芎嗪 (LZ)、尼莫地平的干预作用。方法 建立脐静脉内皮细胞体外培养的方法 ,用放射免疫方法测定ET含量 ,用硝酸还原酶法测定NO的代谢产科NOx含量 ,并观察血性脑脊液和尼莫地平及川芎嗪的作用效果。结果 川芎嗪和尼莫地平可抑制正常体外培养的内皮细胞分泌ET ,而对NO的产生无影响 ;不同时间血性脑脊液均使内皮细胞ET分泌增加 ,NO分泌减少 ;川芎嗪既能减少BCSF引起的ET升高 ,又可抑制NO的下降 ,而尼莫地平仅能抑制ET的增高 ,对NO的变化无明显影响。结论 蛛网膜下腔出血 (SAH)后 ,红细胞溶解破坏 ,释放出氧合血红蛋白 (OxyHb) ,进而通过破坏ET/NO的平衡 ,导致脑血管痉挛 (CVS)。川芎嗪和尼莫地平可通过抑制这一作用而防治CVS。
Objective To observe the effect of blood-borne cerebrospinal fluid (BCSF) on the secretion of endothelin (ET) and nitric oxide (NO) in endothelial cells and the intervention of ligustrazine (LZ) and nimodipine. Methods The method of culturing human umbilical vein endothelial cells in vitro was established. The content of ET was determined by radioimmunoassay. Nitric oxide reductase was used to determine NO metabolites in obstetrics and gynecology. The effects of bloody cerebrospinal fluid and nimodipine and ligustrazine were observed. Results Tetramethylpyrazine and nimodipine could inhibit ET secreted by endothelial cells cultured in vitro, but had no effect on the production of NO. Cerebral CSF at different times increased endotheliocyte ET secretion and NO secretion, while tetramethylpyrazine reduced BCSF ET increased, but also inhibited the decline of NO, while nimodipine only inhibited the increase of ET, NO had no significant effect on the change. Conclusions After subarachnoid hemorrhage (SAH), erythrocyte lysis is destroyed and oxygenated hemoglobin (OxyHb) is released, leading to cerebral vasospasm (CVS) by disrupting the balance of ET / NO. Tetramethylpyrazine and nimodipine can prevent CVS by inhibiting this effect.